Journal: PLoS ONE

Article Title: A Cost-Effective Method to Assemble Biomimetic 3D Cell Culture Platforms

doi: 10.1371/journal.pone.0167116

Figure Lengend Snippet: The top panel shows a graph depicting proliferation of the hBMSCs as expressed by the relative % reduction of the Alamar Blue reagent using the absorbance values in the study group compared to the control group. The lower panel presents inverted microscope images (x10) of adipogenic (top) and osteogenic differentiation (bottom) of hBMSC grown on prototype 1(hAM coated plates) and control culture plate.

Article Snippet: Human bone marrow mesenchymal stem cells (hBMSC) (ATCC ® PCS-500-012, ATCC Inc., USA) were cultured in prototype 1 (prepared using decellularized hAM as described above using a 96-well plate).

Techniques: Control, Inverted Microscopy

Journal: Cell

Article Title: Identification of the Human Skeletal Stem Cell

doi: 10.1016/j.cell.2018.07.029

Figure Lengend Snippet: RESOURCES TABLE

Article Snippet: Anti-human CD34 microbeads (1:100) , Miltenyi Biotec , Cat#130–056-701.

Techniques: Purification, Virus, Recombinant, Electron Microscopy, Saline, Reverse Transcription, Staining, Derivative Assay, Modification, DNA Library Preparation, Immunodetection, Plasmid Preparation, Microarray, Expressing, Software, Inverted Microscopy, Quantitation Assay, Gene Expression

Journal: PLoS ONE

Article Title: Analysis of acute lymphoblastic leukemia drug sensitivity by changes in impedance via stromal cell adherence

doi: 10.1371/journal.pone.0258140

Figure Lengend Snippet: (A) Impedance was measured over 7 days in wells containing OP9 alone (10,000 cells/well), US7 alone (50,000 cells/well) and US7 plated on OP9 feeder cells as indicated (n = 2). When added to the well, OP9 stromal cells adhere to the electrodes and increase impedance (Cell Index) over time. PDX-derived BCP-ALL cells (US7) do not attach to the electrodes and do not affect the measured impedance; however, when co-cultured with OP9, they cause a reduction in impedance. (B) Impedance in co-culture of OP9 cells with different amounts of US7 cells as indicated below the figure (n = 2, triangle = no cells, diamond = 50,000 cells, square = 5,000 cells, circle = 500,000 cells). The reduction in impedance is proportional to the number of added US7 cells. (C) Bright field images of OP9 alone (10,000 cells/well) or OP9 and US7 BCP-ALL cells (50,000 cells/well) 7 days after leukemia cell addition. Dark areas are generated by electrodes blocking the light path in the inverted microscope. Cells are visible in the clear space between electrodes. Scale bar = 100 μm.

Article Snippet: The murine OP9 bone marrow stromal cell line (CRL-2749) and the human HS5 bone marrow stromal cell line (PCS-500-041) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Derivative Assay, Cell Culture, Co-Culture Assay, Generated, Blocking Assay, Inverted Microscopy

Journal: PLoS ONE

Article Title: Analysis of acute lymphoblastic leukemia drug sensitivity by changes in impedance via stromal cell adherence

doi: 10.1371/journal.pone.0258140

Figure Lengend Snippet: Primary (non-cultured) BCP-ALL samples Ph+ BM61, Ph+ BM13 and BM47 were plated (100,000 cells/well) one day after OP9 feeder cells (10,000 cells/well) and impedance was measured over more than 7 days (n = 2). BM13 and BM61 were run on the same plate, whereas BM47 was from a different experiment.

Article Snippet: The murine OP9 bone marrow stromal cell line (CRL-2749) and the human HS5 bone marrow stromal cell line (PCS-500-041) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Analysis of acute lymphoblastic leukemia drug sensitivity by changes in impedance via stromal cell adherence

doi: 10.1371/journal.pone.0258140

Figure Lengend Snippet: (A) US7 cells treated with 100 nM vincristine as indicated (n = 2). (B) Cell Index profile of dose-response to vincristine treatment of OP9+US7. Dotted line indicates the time point used for calculating the IC50 (n = 3 except 1.25nm and 315pm n = 2). (C) Dose-response curve and IC50 calculated from the Cell Index profile in panel B. The IC50 value for vincristine treatment of US7 was 1.87 nM. Statistical analysis was done using one-way ANOVA followed by Dunnett’s multiple comparison test. Error bars represent standard deviation. p values relative to control: ***<0.001; ****<0.0001 (D) ICN13 cells treated with 20 nM vincristine (n = 2). (E) Images of cells indicated in the figure inside the 16-well plate devices, captured after 7 days of treatment with 10 nM (US7) or 20 nM (ICN13) vincristine. Scale bar = 100 μm.

Article Snippet: The murine OP9 bone marrow stromal cell line (CRL-2749) and the human HS5 bone marrow stromal cell line (PCS-500-041) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Comparison, Standard Deviation, Control

Journal: PLoS ONE

Article Title: Analysis of acute lymphoblastic leukemia drug sensitivity by changes in impedance via stromal cell adherence

doi: 10.1371/journal.pone.0258140

Figure Lengend Snippet: (A) Impedance profile of three BCP-ALL cell lines treated with the JAK2 inhibitor ruxolitinib (rux) (n = 3). Dotted lines show the time points used for the calculation of % displacement in panel B (also see ) (B) Percentage impedance signal displacement from treatments with the indicated drug concentrations (n = 3). Statistical analysis was done using one-way ANOVA followed by Dunnett’s multiple comparison test to compare treatment samples versus the OP9+ALL+DMSO control wells. Error bars represent standard deviation. p values relative to control: *<0.05; **<0.01; ***<0.001; ****<0.0001.

Article Snippet: The murine OP9 bone marrow stromal cell line (CRL-2749) and the human HS5 bone marrow stromal cell line (PCS-500-041) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Comparison, Control, Standard Deviation